• Western blot analysis of extracts of Rat kidney, using SPATA5 antibody (STJ11103345) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Mouse pancreas, using SPATA5 antibody (STJ11103345) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of 293T cells, using SPATA5 antibody (STJ11103345) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SPATA5 antibody (228-304) (STJ11103345)

SKU:
STJ11103345

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPATA5 (228-304) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AFG2A
Gene ID: 166378
Uniprot ID: AFG2A_HUMAN
Immunogen Region: 228-304
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 228-304 of human SPATA5 (NP_001304728).
Immunogen Sequence: LELSLQLSQLDLEDTQIPTS RSTPYKPIDDRITNKASDVL LDVTQSPGDGSGLMLEEVTG LKCNFESAREGNEQLTE
Function ATP-dependent chaperone, which plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles. Acts together with AFG2B, AIRIM and CINP. May be involved in morphological and functional mitochondrial transformations during spermatogenesis.
Protein Name Atpase Family Gene 2 Protein Homolog A
Afg2 Aaa Atpase Homolog A
Ribosome Biogenesis Protein Spata5
Spermatogenesis-Associated Factor Protein
Spermatogenesis-Associated Protein 5
Cellular Localisation Cytoplasm
Mitochondrion
Cytoskeleton
Spindle
Alternative Antibody Names Anti-Atpase Family Gene 2 Protein Homolog A antibody
Anti-Afg2 Aaa Atpase Homolog A antibody
Anti-Ribosome Biogenesis Protein Spata5 antibody
Anti-Spermatogenesis-Associated Factor Protein antibody
Anti-Spermatogenesis-Associated Protein 5 antibody
Anti-AFG2A antibody
Anti-SPAF antibody
Anti-SPATA5 antibody

Information sourced from Uniprot.org

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