| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SPAG8 |
| Gene ID: | 26206 |
| Uniprot ID: | SPAG8_HUMAN |
| Immunogen Region: | 220-420 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1). |
| Immunogen Sequence: | LVADRVPNYTSWSQHCPWEP QKQPPWEFLQVLEPGARGLW KPPDIKGKLMVCYETLPRGQ CLLYNWEEERATNHLDQVPS MQDGSESFFFRHGHRGLLTM QLKSPMPSSTTQKDSYQPPG NVYWPLRGKREAMLEMLLQH QICKEVQAEQEPTRKLFEVE SVTHHDYRMELAQAGTPAPT KPHDYRQEQPETFWIQRAPQ L |
| Tissue Specificity | Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. Expressed in airway epithelial cells. |
| Function | Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau. Involved in the acrosome reaction and in binding of sperm to the zona pellucida. Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. May play a role in fertility and microtubule formation through interaction with RANBP9. |
| Protein Name | Sperm-Associated Antigen 8 Hsd-1 Sperm Membrane Protein 1 Smp-1 Sperm Membrane Protein Bs-84 |
| Cellular Localisation | Cytoplasm Nucleus Cytoplasmic Vesicle Secretory Vesicle Acrosome Cytoskeleton Microtubule Organizing Center Spindle Cilium Axoneme Flagellum Axoneme In Mature Sperm Cells Detected In The Acrosomal Region Of The Head And In The Middle Piece Of The Tail Localized To The Nucleus And Cytoplasm Of Spermatocytes And Round Spermatids While In Elongating Spermatids Expressed In The Cytoplasm But Not In The Nucleus During The Cell Cycle Localized On The Microtubule-Organizing Center (Mtoc) During Prophase In Metaphase Extends Along Spindle Microtubules In Anaphase Detected On The Astral Microtubules And Mid-Zone In Telophase Remains At The Mid-Zone After Cytokinesis Returns To The Mtoc Microtubule Inner Protein (Mip) Part Of The Dynein-Decorated Doublet Microtubules (Dmts) In Cilia Axoneme |
| Alternative Antibody Names | Anti-Sperm-Associated Antigen 8 antibody Anti-Hsd-1 antibody Anti-Sperm Membrane Protein 1 antibody Anti-Smp-1 antibody Anti-Sperm Membrane Protein Bs-84 antibody Anti-SPAG8 antibody |
Information sourced from Uniprot.org