• Western blot analysis of extracts of various cell lines, using SPAG8 antibody (STJ113600) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using SPAG8 Rabbit polyclonal antibody (STJ113600) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPAG8 antibody (220-420) (STJ113600)

SKU:
STJ113600

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SPAG8 (220-420) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SPAG8
Gene ID: 26206
Uniprot ID: SPAG8_HUMAN
Immunogen Region: 220-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SPAG8 (NP_758516.1).
Immunogen Sequence: LVADRVPNYTSWSQHCPWEP QKQPPWEFLQVLEPGARGLW KPPDIKGKLMVCYETLPRGQ CLLYNWEEERATNHLDQVPS MQDGSESFFFRHGHRGLLTM QLKSPMPSSTTQKDSYQPPG NVYWPLRGKREAMLEMLLQH QICKEVQAEQEPTRKLFEVE SVTHHDYRMELAQAGTPAPT KPHDYRQEQPETFWIQRAPQ L
Tissue Specificity Expressed in testis (germ cells), but not in liver, kidney, prostate and small intestine. Expressed in airway epithelial cells.
Function Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating. Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau. Involved in the acrosome reaction and in binding of sperm to the zona pellucida. Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis. May play a role in fertility and microtubule formation through interaction with RANBP9.
Protein Name Sperm-Associated Antigen 8
Hsd-1
Sperm Membrane Protein 1
Smp-1
Sperm Membrane Protein Bs-84
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Cytoskeleton
Microtubule Organizing Center
Spindle
Cilium Axoneme
In Mature Sperm Cells
Detected In The Acrosomal Region Of The Head And In The Middle Piece Of The Tail
Localized To The Nucleus And Cytoplasm Of Spermatocytes And Round Spermatids While
In Elongating Spermatids
Expressed In The Cytoplasm But Not In The Nucleus
During The Cell Cycle
Localized On The Microtubule-Organizing Center (Mtoc) During Prophase
In Metaphase
Extends Along Spindle Microtubules
In Anaphase
Detected On The Astral Microtubules And Mid-Zone
In Telophase
Remains At The Mid-Zone
After Cytokinesis
Returns To The Mtoc
Microtubule Inner Protein (Mip) Part Of The Dynein-Decorated Doublet Microtubules (Dmts) In Cilia Axoneme
Alternative Antibody Names Anti-Sperm-Associated Antigen 8 antibody
Anti-Hsd-1 antibody
Anti-Sperm Membrane Protein 1 antibody
Anti-Smp-1 antibody
Anti-Sperm Membrane Protein Bs-84 antibody
Anti-SPAG8 antibody

Information sourced from Uniprot.org

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