Anti-SOX2 antibody [2A10-F11-F12] (STJA0032351)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenPurified recombinant mouse Sox2 protein
General Information
| Short Description | Mouse monoclonal anti-SOX2 for use in WB, IHC-P, ICC, IF and FC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/FC |
| Host | Mouse |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2A10-F11-F12 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100ICC 1:150FC 1:100 |
| Formulation | Liquid in PBS with 0.09% (W/V) sodium azide |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | SOX2 |
| Gene ID | 6657 |
| Uniprot ID | SOX2_HUMAN |
| Immunogen | Purified recombinant mouse Sox2 protein |
Additional Info
| Post Translational Modifications | Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation. Ubiquitinated by WWP2, leading to proteasomal degradation. |
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Binds to the proximal enhancer region of NANOG. Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. May function as a switch in neuronal development. |
| Protein Name | Transcription Factor Sox-2 |
| Database Links | Reactome: R-HSA-2892245Reactome: R-HSA-2892247Reactome: R-HSA-3769402Reactome: R-HSA-452723Reactome: R-HSA-6785807Reactome: R-HSA-8986944Reactome: R-HSA-9754189Reactome: R-HSA-9823739Reactome: R-HSA-9832991Reactome: R-HSA-9834899Reactome: R-HSA-9856649Reactome: R-HSA-9926550 |
| Cellular Localisation | Nucleus SpeckleCytoplasmNucleusAcetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic DelocalizationColocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)Colocalizes With Sox6 In SpecklesColocalizes With Caml In The NucleusNuclear Import Is Facilitated By Xpo4A Protein That Usually Acts As A Nuclear Export Signal Receptor |
| Alternative Antibody Names | Anti-Transcription Factor Sox-2 antibodyAnti-SOX2 antibody |
Information sourced from Uniprot.org