• Immunofluorescence analysis of U2OS cells using SOD2 Rabbit polyclonal antibody (STJ11104554) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SOD2 Rabbit polyclonal antibody (STJ11104554) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using SOD2 Rabbit polyclonal antibody (STJ11104554) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SOD2 in paraffin-embedded mouse kidney using SOD2 Rabbit polyclonal antibody (STJ11104554) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SOD2 in paraffin-embedded human colon using SOD2 Rabbit polyclonal antibody (STJ11104554) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SOD2 in paraffin-embedded rat kidney using SOD2 Rabbit polyclonal antibody (STJ11104554) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and SOD2 knockout (KO) 293T (KO) cells, using SOD2 Rabbit polyclonal antibody (STJ11104554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates, using SOD2 Rabbit polyclonal antibody (STJ11104554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis lysates from HepG2, using SOD2 Rabbit polyclonal antibody (STJ11104554) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SOD2 antibody (25-222) (STJ11104554)

SKU:
STJ11104554

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Superoxide Dismutase Mn-Mitochondrial (25-222) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOD2
Gene ID: 6648
Uniprot ID: SODM_HUMAN
Immunogen Region: 25-222
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-222 of human SOD2 (NP_000627.2).
Immunogen Sequence: KHSLPDLPYDYGALEPHINA QIMQLHHSKHHAAYVNNLNV TEEKYQEALAKGDVTAQIAL QPALKFNGGGHINHSIFWTN LSPNGGGEPKGELLEAIKRD FGSFDKFKEKLTAASVGVQG SGWGWLGFNKERGHLQIAAC PNQDPLQGTTGLIPLLGIDV WEHAYYLQYKNVRPDYLKAI WNVINWENVTERYMACKK
Post Translational Modifications Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting. Polyubiquitinated.leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Protein Name Superoxide Dismutase Mn - Mitochondrial
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-3299685
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9615017
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Superoxide Dismutase Mn - Mitochondrial antibody
Anti-SOD2 antibody

Information sourced from Uniprot.org

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