• Western blot analysis of various lysates, using SOD2 rabbit monoclonal antibody (STJ11101707) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Western blot analysis of extracts from wild type (WT) and SOD2 knockout (KO) 293T (KO) cells, using SOD2 rabbit monoclonal antibody (STJ11101707) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using SOD2 antibody (STJ11101707) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal using SOD2 antibody (STJ11101707) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Confocal imaging of A549 cells using SOD2 rabbit monoclonal antibody (STJ11101707, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-SOD2 antibody (1-100) [S7MR] (STJ11101707)

SKU:
STJ11101707

£146.50 - £642.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:8000
IHC-P:1:200-1:2000
IF/ICC:1:200-1:800
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SOD2
Gene ID: 6648
Uniprot ID: SODM_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SOD2 (P04179).
Immunogen Sequence: MLSRAVCGTSRQLAPVLGYL GSRQKHSLPDLPYDYGALEP HINAQIMQLHHSKHHAAYVN NLNVTEEKYQEALAKGDVTA QIALQPALKFNGGGHINHSI
Post Translational Modifications Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting. Polyubiquitinated.leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.
Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Protein Name Superoxide Dismutase Mn - Mitochondrial
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-3299685
Reactome: R-HSA-8862803
Reactome: R-HSA-8950505
Reactome: R-HSA-9615017
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Superoxide Dismutase Mn - Mitochondrial antibody
Anti-SOD2 antibody

Information sourced from Uniprot.org

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