• Western blot analysis of extracts of various cell lines, using SNRPD1 antibody (STJ116704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using SNRPD1 rabbit polyclonal antibody (STJ116704) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using SNRPD1 rabbit polyclonal antibody (STJ116704) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using SNRPD1 rabbit polyclonal antibody (STJ116704) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using SNRPD1 rabbit polyclonal antibody (STJ116704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using SNRPD1 rabbit polyclonal antibody (STJ116704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SNRPD1 antibody (1-96) (STJ116704)

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STJ116704

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNRPD1 (1-96) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNRPD1
Gene ID: 6632
Uniprot ID: SMD1_HUMAN
Immunogen Region: 1-96
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-96 of human SNRPD1 (NP_008869.1).
Immunogen Sequence: MKLVRFLMKLSHETVTIELK NGTQVHGTITGVDVSMNTHL KAVKMTLKNREPVQLETLSI RGNNIRYFILPDSLPLDTLL VDVEPKVKSKKREAVA
Post Translational Modifications Methylated on arginine residues by PRMT5 and PRMT7.probable asymmetric dimethylation which is required for assembly and biogenesis of snRNPs.
Function Plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs. May act as a charged protein scaffold to promote snRNP assembly or strengthen snRNP-snRNP interactions through non-specific electrostatic contacts with RNA (Probable).
Protein Name Small Nuclear Ribonucleoprotein Sm D1
Sm-D1
Sm-D Autoantigen
Snrnp Core Protein D1
Database Links Reactome: R-HSA-191859
Reactome: R-HSA-72163
Reactome: R-HSA-72165
Reactome: R-HSA-9754678
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Smn-Mediated Assembly Into Core Snrnps Occurs In The Cytosol Before Smn-Mediated Transport To The Nucleus To Be Included In Spliceosomes
Alternative Antibody Names Anti-Small Nuclear Ribonucleoprotein Sm D1 antibody
Anti-Sm-D1 antibody
Anti-Sm-D Autoantigen antibody
Anti-Snrnp Core Protein D1 antibody
Anti-SNRPD1 antibody

Information sourced from Uniprot.org

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