| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SNAPIN (1-136) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:200-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SNAPIN |
| Gene ID: |
23557 |
| Uniprot ID: |
SNAPN_HUMAN |
| Immunogen Region: |
1-136 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human SNAPIN (NP_036569.1). |
| Immunogen Sequence: |
MAGAGSAAVSGAGTPVAGPT GRDLFAEGLLEFLRPAVQQL DSHVHAVRESQVELREQIDN LATELCRINEDQKVALDLDP YVKKLLNARRRVVLVNNILQ NAQERLRRLNHSVAKETARR RAMLDSGIYPPGSPGK |
| Tissue Specificity | Expressed in male germ cells of adult testis (at protein level). |
| Post Translational Modifications | Phosphorylated by CSNK1D/CK1. Phosphorylated by PKD, phosphorylation controls SNAPIN protein stability. |
| Function | Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor. |
| Protein Name | Snare-Associated Protein SnapinBiogenesis Of Lysosome-Related Organelles Complex 1 Subunit 7Bloc-1 Subunit 7Synaptosomal-Associated Protein 25-Binding ProteinSnap-Associated Protein |
| Database Links | Reactome: R-HSA-432722 |
| Cellular Localisation | MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmCytosolPerinuclear RegionGolgi Apparatus MembraneLysosome MembraneCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembraneColocalizes With Nanos1 And Pum2 In The Perinuclear Region Of Germ Cells |
| Alternative Antibody Names | Anti-Snare-Associated Protein Snapin antibodyAnti-Biogenesis Of Lysosome-Related Organelles Complex 1 Subunit 7 antibodyAnti-Bloc-1 Subunit 7 antibodyAnti-Synaptosomal-Associated Protein 25-Binding Protein antibodyAnti-Snap-Associated Protein antibodyAnti-SNAPIN antibodyAnti-BLOC1S7 antibodyAnti-SNAP25BP antibodyAnti-SNAPAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and SNAPIN knockout (KO) HeLa cells, using [KO Validated] SNAPIN Rabbit polyclonal antibody (STJ11100856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89790/358261/STJ11100856_1__86855.1713122601.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SNAPIN knockout (KO) HeLa cells, using [KO Validated] SNAPIN Rabbit polyclonal antibody (STJ11100856) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
