• Immunohistochemistry analysis of paraffin-embedded rat kidney using SNAPIN Rabbit polyclonal antibody (STJ112332) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using SNAPIN Rabbit polyclonal antibody (STJ112332) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SNAPIN antibody (STJ112332) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-SNAPIN antibody (1-136) (STJ112332)

SKU:
STJ112332

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SNAPIN (1-136) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SNAPIN
Gene ID: 23557
Uniprot ID: SNAPN_HUMAN
Immunogen Region: 1-136
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-136 of human SNAPIN (NP_036569.1).
Immunogen Sequence: MAGAGSAAVSGAGTPVAGPT GRDLFAEGLLEFLRPAVQQL DSHVHAVRESQVELREQIDN LATELCRINEDQKVALDLDP YVKKLLNARRRVVLVNNILQ NAQERLRRLNHSVAKETARR RAMLDSGIYPPGSPGK
Tissue Specificity Expressed in male germ cells of adult testis (at protein level).
Post Translational Modifications Phosphorylated by CSNK1D/CK1. Phosphorylated by PKD, phosphorylation controls SNAPIN protein stability.
Function Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.
Protein Name Snare-Associated Protein Snapin
Biogenesis Of Lysosome-Related Organelles Complex 1 Subunit 7
Bloc-1 Subunit 7
Synaptosomal-Associated Protein 25-Binding Protein
Snap-Associated Protein
Database Links Reactome: R-HSA-432722
Cellular Localisation Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytosol
Perinuclear Region
Golgi Apparatus Membrane
Lysosome Membrane
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Colocalizes With Nanos1 And Pum2 In The Perinuclear Region Of Germ Cells
Alternative Antibody Names Anti-Snare-Associated Protein Snapin antibody
Anti-Biogenesis Of Lysosome-Related Organelles Complex 1 Subunit 7 antibody
Anti-Bloc-1 Subunit 7 antibody
Anti-Synaptosomal-Associated Protein 25-Binding Protein antibody
Anti-Snap-Associated Protein antibody
Anti-SNAPIN antibody
Anti-BLOC1S7 antibody
Anti-SNAP25BP antibody
Anti-SNAPAP antibody

Information sourced from Uniprot.org

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