• Biotinylated STJ70695 (0. 1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-SNAP25, Biotinylated antibody (C-Term) {Biotin} (STJ73237)

SKU:
STJ73237-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SNAP25, Biotinylated (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:64000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SNAP25
Gene ID: 6616
Uniprot ID: SNP25_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003072.2; NP_570824.1
Specificity: This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, N
Immunogen Sequence: DEANQRATKMLGSG.
Post Translational Modifications Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release. C.botulinum type C only rarely infects humans. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release.
Function t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.
Protein Name Synaptosomal-Associated Protein 25
Snap-25
Super Protein
Sup
Synaptosomal-Associated 25 Kda Protein
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-422356
Reactome: R-HSA-449836
Reactome: R-HSA-5250968
Reactome: R-HSA-5250971
Reactome: R-HSA-5250992
Reactome: R-HSA-6798695
Reactome: R-HSA-888590
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasm
Perinuclear Region
Cell Membrane
Lipid-Anchor
Synapse
Synaptosome
Photoreceptor Inner Segment
Membrane Association Requires Palmitoylation
Expressed Throughout Cytoplasm
Concentrating At The Perinuclear Region
Colocalizes With Kcnb1 At The Cell Membrane
Colocalizes With Plcl1 At The Cell Membrane
Alternative Antibody Names Anti-Synaptosomal-Associated Protein 25 antibody
Anti-Snap-25 antibody
Anti-Super Protein antibody
Anti-Sup antibody
Anti-Synaptosomal-Associated 25 Kda Protein antibody
Anti-SNAP25 antibody
Anti-SNAP antibody

Information sourced from Uniprot.org

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