| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SMYD2 |
| Gene ID: | 56950 |
| Uniprot ID: | SMYD2_HUMAN |
| Immunogen Region: | 1-270 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SMYD2 (NP_064582.2). |
| Immunogen Sequence: | MRAEGLGGLERFCSPGKGRG LRALQPFQVGDLLFSCPAYA YVLTVNERGNHCEYCFTRKE GLSKCGRCKQAFYCNVECQK EDWPMHKLECSPMVVFGENW NPSETVRLTARILAKQKIHP ERTPSEKLLAVKEFESHLDK LDNEKKDLIQSDIAALHHFY SKHLGFPDNDSLVVLFAQVN CNGFTIEDEELSHLGSAIFP DVALMNHSCCPNVIVTYKGT LAEVRAVQEIKPGEEVFTS |
| Function | Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically trimethylates histone H3 'Lys-4' (H3K4me3) in vivo. The activity requires interaction with HSP90alpha. Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'. |
| Protein Name | N-Lysine Methyltransferase Smyd2Hskm-BHistone Methyltransferase Smyd2Lysine N-Methyltransferase 3cSet And Mynd Domain-Containing Protein 2 |
| Database Links | Reactome: R-HSA-3214841Reactome: R-HSA-6804760 |
| Cellular Localisation | CytoplasmCytosolNucleus |
| Alternative Antibody Names | Anti-N-Lysine Methyltransferase Smyd2 antibodyAnti-Hskm-B antibodyAnti-Histone Methyltransferase Smyd2 antibodyAnti-Lysine N-Methyltransferase 3c antibodyAnti-Set And Mynd Domain-Containing Protein 2 antibodyAnti-SMYD2 antibodyAnti-KMT3C antibody |
Information sourced from Uniprot.org