• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SMOC1 antibody (STJ11103334) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Mouse liver, using SMOC1 antibody (STJ11103334) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SMOC1 antibody (27-250) (STJ11103334)

SKU:
STJ11103334

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SMOC1 (27-250) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SMOC1
Gene ID: 64093
Uniprot ID: SMOC1_HUMAN
Immunogen Region: 27-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-250 of human SMOC1 (NP_071420.1).
Immunogen Sequence: HRTTGPRFLISDRDPQCNLH CSRTQPKPICASDGRSYESM CEYQRAKCRDPTLGVVHRGR CKDAGQSKCRLERAQALEQA KKPQEAVFVPECGEDGSFTQ VQCHTYTGYCWCVTPDGKPI SGSSVQNKTPVCSGSVTDKP LSQGNSGRKDDGSKPTPTME TQPVFDGDEITAPTLWIKHL VIKDSKLNNTNIRNSEKVYS CDQERQSALEEAQQNPREGI VIPE
Tissue Specificity Widely expressed in many tissues with a strongest signal in ovary. No expression in spleen.
Post Translational Modifications Glycosylated.
Function Plays essential roles in both eye and limb development. Probable regulator of osteoblast differentiation.
Protein Name Sparc-Related Modular Calcium-Binding Protein 1
Secreted Modular Calcium-Binding Protein 1
Smoc-1
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Basement Membrane
In Or Around The Basement Membrane
Alternative Antibody Names Anti-Sparc-Related Modular Calcium-Binding Protein 1 antibody
Anti-Secreted Modular Calcium-Binding Protein 1 antibody
Anti-Smoc-1 antibody
Anti-SMOC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance