| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Smad7 (220-420) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMAD7 |
| Gene ID: |
4092 |
| Uniprot ID: |
SMAD7_HUMAN |
| Immunogen Region: |
220-420 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 220-420 of human Smad7 (NP_005895.1). |
| Immunogen Sequence: |
KPTADCPDAVPSSAETGGTN YLAPGGLSDSQLLLEPGDRS HWCVVAYWEEKTRVGRLYCV QEPSLDIFYDLPQGNGFCLG QLNSDNKSQLVQKVRSKIGC GIQLTREVDGVWVYNRSSYP IFIKSATLDNPDSRTLLVHK VFPGFSIKAFDYEKAYSLQR PNDHEFMQQPWTGFTVQISF VKGWGQCYTRQFISSCPCWL E |
| Tissue Specificity | Ubiquitous with higher expression in the lung and vascular endothelium. |
| Post Translational Modifications | Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling.however it affects its ability to regulate transcription. Phosphorylated by PDPK1. Ubiquitinated by WWP1. Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1.which promotes its degradation. Acetylation prevents ubiquitination and degradation mediated by SMURF1. |
| Function | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members.has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 7Mad Homolog 7Mothers Against Dpp Homolog 7Mothers Against Decapentaplegic Homolog 8Mad Homolog 8Mothers Against Dpp Homolog 8Smad Family Member 7Smad 7Smad7Hsmad7 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-2173788Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-5689603Reactome: R-HSA-5689880Reactome: R-HSA-877300 |
| Cellular Localisation | NucleusCytoplasmInteraction With Nedd4l Or Rnf111 Induces Translocation From The Nucleus To The CytoplasmTgf-Beta Stimulates Its Translocation From The Nucleus To The CytoplasmPdpk1 Inhibits Its Translocation From The Nucleus To The Cytoplasm In Response To Tgf-Beta |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 7 antibodyAnti-Mad Homolog 7 antibodyAnti-Mothers Against Dpp Homolog 7 antibodyAnti-Mothers Against Decapentaplegic Homolog 8 antibodyAnti-Mad Homolog 8 antibodyAnti-Mothers Against Dpp Homolog 8 antibodyAnti-Smad Family Member 7 antibodyAnti-Smad 7 antibodyAnti-Smad7 antibodyAnti-Hsmad7 antibodyAnti-SMAD7 antibodyAnti-MADH7 antibodyAnti-MADH8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
