| Host: |
Mouse |
| Applications: |
WB/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Mothers against decapentaplegic homolog 6 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
5H3 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SMAD6 |
| Gene ID: |
4091 |
| Uniprot ID: |
SMAD6_HUMAN |
| Specificity: |
Smad6 Monoclonal Antibody detects endogenous levels of Smad6 protein. |
| Immunogen: |
Purified recombinant fragment of human Smad6 expressed in E. Coli. |
| Post Translational Modifications | Phosphorylated by BMP type 1 receptor kinase and by PRKX. Monoubiquitinated at Lys-173 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation. Ubiquitinated by WWP1. Ubiquitinated by ARK2C, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling. Arginine methylation by PRMT1, which is recruited by BMPR2, initiates BMP-Induced signaling and induces dissociation from the BMPR1B receptor at the cell surface leading to derepress downstream Smad1/Smad5 signaling. |
| Function | Transforming growth factor-beta superfamily receptors signaling occurs through the Smad family of intracellular mediators. SMAD6 is an inhibitory Smad (i-Smad) that negatively regulates signaling downstream of type I transforming growth factor-beta. Acts as a mediator of TGF-beta and BMP anti-inflammatory activities. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of pro-inflammatory genes. Blocks the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions. |
| Protein Name | Mothers Against Decapentaplegic Homolog 6Mad Homolog 6Mothers Against Dpp Homolog 6Smad Family Member 6Smad 6Smad6Hsmad6 |
| Database Links | Reactome: R-HSA-201451Reactome: R-HSA-8941326 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 6 antibodyAnti-Mad Homolog 6 antibodyAnti-Mothers Against Dpp Homolog 6 antibodyAnti-Smad Family Member 6 antibodyAnti-Smad 6 antibodyAnti-Smad6 antibodyAnti-Hsmad6 antibodyAnti-SMAD6 antibodyAnti-MADH6 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
