![Immunohistochemistry analysis of paraffin-embedded human liver cancer using [KO Validated] Smad4 rabbit monoclonal antibody (STJ11101653) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90567/360267/STJ11101653_IHC_4__10941.1713124903.jpg?c=1 "Immunohistochemistry analysis of paraffin-embedded human liver cancer using [KO Validated] Smad4 rabbit monoclonal antibody (STJ11101653) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.")
![Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using [KO Validated] Smad4 rabbit monoclonal antibody (STJ11101653) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/90567/360269/STJ11101653_IP_6__77193.1713124904.png?c=1 "Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using [KO Validated] Smad4 rabbit monoclonal antibody (STJ11101653) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.")
Anti-SMAD4 antibody (320-552) [S3MR] (STJ11101653)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal Smad4 (320-552) antibody for use in WB, IHC-P, IP, ELISA, ChIP and CUT&Tag in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IP/ELISA/ChIP/CUT&Tag |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S3MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requiremen |
| Formulation | PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SMAD4 |
| Gene ID | 4089 |
| Uniprot ID | SMAD4_HUMAN |
| Immunogen Region | 320-552 |
| Immunogen Sequence | PEYWCSIAYFEMDVQVGETF KVPSSCPIVTVDGYVDPSGG DRFCLGQLSNVHRTEAIERA RLHIGKGVQLECKGEGDVWV RCLSDHAVFVQSYYLDREAG RAPGDAVHKIYPSAYIKVFD LRQCHRQMQQQAATAQAAAA AQAAAVAGNIPGPGSVGGIA PAISLSAAAGIGVDDLRRLC ILRMSFVKGWGPDYPRQSIK ETPCWIEIHLHRALQLLDEV LHTMPIADPQPLD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 320-552 of human Smad4 (NP_005350.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by PDPK1. Monoubiquitinated on Lys-519 by E3 ubiquitin-protein ligase TRIM33. Monoubiquitination hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade. Deubiquitination by USP9X restores its competence to mediate TGF-beta signaling. |
| Function | In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site.required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Protein Name | Mothers Against Decapentaplegic Homolog 4Mad Homolog 4Mothers Against Dpp Homolog 4Deletion Target In Pancreatic Carcinoma 4Smad Family Member 4Smad 4Smad4Hsmad4 |
| Database Links | Reactome: R-HSA-1181150Reactome: R-HSA-1502540Reactome: R-HSA-201451Reactome: R-HSA-2173789Reactome: R-HSA-2173795Reactome: R-HSA-2173796Reactome: R-HSA-3311021Reactome: R-HSA-3315487Reactome: R-HSA-452723Reactome: R-HSA-5689880Reactome: R-HSA-8941326Reactome: R-HSA-8941855Reactome: R-HSA-8952158Reactome: R-HSA-9615017Reactome: R-HSA-9617828Reactome: R-HSA-9733709Reactome: R-HSA-9735871Reactome: R-HSA-9754189Reactome: R-HSA-9823730Reactome: R-HSA-9839394Reactome: R-HSA-9844594 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic In The Absence Of LigandMigrates To The Nucleus When Complexed With R-SmadPdpk1 Prevents Its Nuclear Translocation In Response To Tgf-Beta |
| Alternative Antibody Names | Anti-Mothers Against Decapentaplegic Homolog 4 antibodyAnti-Mad Homolog 4 antibodyAnti-Mothers Against Dpp Homolog 4 antibodyAnti-Deletion Target In Pancreatic Carcinoma 4 antibodyAnti-Smad Family Member 4 antibodyAnti-Smad 4 antibodyAnti-Smad4 antibodyAnti-Hsmad4 antibodyAnti-SMAD4 antibodyAnti-DPC4 antibodyAnti-MADH4 antibody |
Information sourced from Uniprot.org