• STJ70945 (4µg/ml) staining of paraffin embedded Human Spinal Cord. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Anti-SLIT2 antibody (Internal) (STJ70945)

SKU:
STJ70945-100

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Host: Goat
Applications: Pep-ELISA/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SLIT2 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-Recommended concentration, 4-8µg/ml
ELISA-antibody detection limit dilution 1:1000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: SLIT2
Gene ID: 9353
Uniprot ID: SLIT2_HUMAN
Immunogen Region: Internal
Accession Number: NP_004778.1
Immunogen Sequence: DDCQDNKCKNGAH
Function Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
Protein Name Slit Homolog 2 Protein
Slit-2 Cleaved Into - Slit Homolog 2 Protein N-Product - Slit Homolog 2 Protein C-Product
Database Links Reactome: R-HSA-373752
Reactome: R-HSA-376176
Reactome: R-HSA-428540
Reactome: R-HSA-428542
Reactome: R-HSA-428543
Reactome: R-HSA-428890
Reactome: R-HSA-8985586
Reactome: R-HSA-9010553
Cellular Localisation Secreted
The C-Terminal Cleavage Protein Is More Diffusible Than The Larger N-Terminal Protein That Is More Tightly Cell Associated
Alternative Antibody Names Anti-Slit Homolog 2 Protein antibody
Anti-Slit-2 Cleaved Into - Slit Homolog 2 Protein N-Product - Slit Homolog 2 Protein C-Product antibody
Anti-SLIT2 antibody
Anti-SLIL3 antibody

Information sourced from Uniprot.org