• Western blot analysis of extracts of various cell lines, using SLC9A6 antibody (STJ110486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using SLC9A6 antibody (STJ110486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SLC9A6 antibody (542-701) (STJ110486)

SKU:
STJ110486

$151.88 - $705.38
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Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC9A6 (542-701) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC9A6
Gene ID: 10479
Uniprot ID: SL9A6_HUMAN
Immunogen Region: 542-701
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 542-701 of human SLC9A6 (NP_001036002.1).
Immunogen Sequence: VDSDQEHLGVPENERRTTKA ESAWLFRMWYNFDHNYLKPL LTHSGPPLTTTLPACCGPIA RCLTSPQAYENQEQLKDDDS DLILNDGDISLTYGDSTVNT EPATSSAPRRFMGNSSEDAL DRELAFGDHELVIRGTRLVL PMDDSEPPLNLLDNTRHGPA
Tissue Specificity Ubiquitous. High expression in brain, skeletal muscle, and heart, but is also detected at lower levels in most other tissues.
Post Translational Modifications Ubiquitinated (in vitro). Glycosylated.
Function Endosomal Na(+), K(+)/H(+) antiporter. Mediates the electroneutral exchange of endosomal luminal H(+) for a cytosolic Na(+) or K(+). By facilitating proton efflux, SLC9A6 counteracts the acidity generated by vacuolar (V)-ATPase, thereby limiting luminal acidification. Responsible for alkalizing and maintaining the endosomal pH, and consequently in, e.g., endosome maturation and trafficking of recycling endosomal cargo. Plays a critical role during neurodevelopment by regulating synaptic development and plasticity. Implicated in the maintenance of cell polarity in a manner that is dependent on its ability to modulate intravesicular pH. Regulates intracelular pH in some specialized cells, osteoclasts and stereocilia where this transporter localizes to the plasma membrane.
Protein Name Sodium/Hydrogen Exchanger 6
Na(+/H(+ Exchanger 6
Nhe-6
Solute Carrier Family 9 Member 6
Database Links Reactome: R-HSA-425986
Reactome: R-HSA-5619092
Cellular Localisation Endosome Membrane
Multi-Pass Membrane Protein
Recycling Endosome Membrane
Early Endosome Membrane
Late Endosome Membrane
Cell Membrane
Present Predominantly In The Recycling Compartments Including Early And Recycling Endosomes
But Undergoes Plasma Membrane Localization During Vesicular Recycling
Which Is Enhanced Upon Certain Stimuli
Such As Hypoxia
Has A Major Plasmalemmal Distribution In A Few Specialized Cells
Such As In Vestibular Hair Bundles And Osteoblasts
Alternative Antibody Names Anti-Sodium/Hydrogen Exchanger 6 antibody
Anti-Na(+/H(+ Exchanger 6 antibody
Anti-Nhe-6 antibody
Anti-Solute Carrier Family 9 Member 6 antibody
Anti-SLC9A6 antibody
Anti-KIAA0267 antibody
Anti-NHE6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance