• Western blot analysis of extracts of various cell lines, using SLC22A11 antibody (STJ110126) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SLC22A11 antibody (40-140) (STJ110126)

SKU:
STJ110126

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC22A11 (40-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC22A11
Gene ID: 55867
Uniprot ID: S22AB_HUMAN
Immunogen Region: 40-140
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-140 of human SLC22A11 (NP_060954.1).
Immunogen Sequence: FSAAIPGHRCWTHMLDNGSA VSTNMTPKALLTISIPPGPN QGPHQCRRFRQPQWQLLDPN ATATSWSEADTEPCVDGWVY DRSVFTSTIVAKWDLVCSSQ G
Tissue Specificity Expressed in placental trophoblasts, syncytiotrophoblast and cytotrophoblast. Also located in the proximal tubules in kidneys.
Post Translational Modifications N-glycosylated. Contains several complex-type N-glycans.
Function Antiporter that mediates the transport of conjugated steroids and other specific organic anions at the basal membrane of syncytiotrophoblast and at the apical membrane of proximal tubule epithelial cells, in exchange for anionic compounds. May be responsible for placental absorption of fetal-derived steroid sulfates such as estrone sulfate (E1S) and the steroid hormone precursor dehydroepiandrosterone sulfate (DHEA-S), as well as clearing waste products and xenobiotics from the fetus. Maybe also be involved in placental urate homeostasis. Facilitates the renal reabsorption of organic anions such as urate and derived steroid sulfates. Organic anion glutarate acts as conteranion for E1S renal uptake. Possible transport mode may also include DHEA-S/E1S exchange. Also interacts with inorganic anions such as chloride and hydroxyl ions, therefore possible transport modes may include E1S/Cl(-), E1S/OH(-), urate/Cl(-) and urate/OH(-). Also mediates the transport of prostaglandin E2 (PGE2) and prostaglandin F2-alpha (PGF2-alpha) and may be involved in their renal excretion. Also able to uptake anionic drugs, diuretics, bile salts and ochratoxin A. Mediates the unidirectional efflux of glutamate and aspartate. Glutamate efflux down its transmembrane gradient may drive SLC22A11/OAT4-mediated placental uptake of E1S.
Protein Name Solute Carrier Family 22 Member 11
Organic Anion Transporter 4
Oat4
Organic Anion -Dicarboxylate Exchanger Oat4
Database Links Reactome: R-HSA-561048
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Basal Cell Membrane
Expressed At The Apical Membrane Of The Proximal Tubule Epithelial Cells
Expressed At The Basal Membrane Of The Syncytiotrophoblast
Alternative Antibody Names Anti-Solute Carrier Family 22 Member 11 antibody
Anti-Organic Anion Transporter 4 antibody
Anti-Oat4 antibody
Anti-Organic Anion -Dicarboxylate Exchanger Oat4 antibody
Anti-SLC22A11 antibody
Anti-OAT4 antibody

Information sourced from Uniprot.org

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