• Immunofluorescence analysis of mouse brain cells using SLC17A7 Rabbit polyclonal antibody (STJ114745) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-SY5Y cells using SLC17A7 Rabbit polyclonal antibody (STJ114745) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of U-87MG cells, using SLC17A7 antibody (STJ114745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SLC17A7 antibody (461-560) (STJ114745)

SKU:
STJ114745

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC17A7 (461-560) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC17A7
Gene ID: 57030
Uniprot ID: VGLU1_HUMAN
Immunogen Region: 461-560
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 461-560 of human SLC17A7 (NP_064705.1).
Immunogen Sequence: KHKTREEWQYVFLIASLVHY GGVIFYGVFASGEKQPWAEP EEMSEEKCGFVGHDQLAGSD DSEMEDEAEPPGAPPAPPPS YGATHSTFQPPRPPPPVRDY
Tissue Specificity Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
Function Multifunctional transporter that transports L-glutamate as well as multiple ions such as chloride, proton, potassium, sodium and phosphate. At the synaptic vesicle membrane, mainly functions as an uniporter which transports preferentially L-glutamate but also phosphate from the cytoplasm into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. The L-glutamate or phosphate uniporter activity is electrogenic and is driven by the proton electrochemical gradient, mainly by the electrical gradient established by the vacuolar H(+)-ATPase across the synaptic vesicle membrane. In addition, functions as a chloride channel that allows a chloride permeation through the synaptic vesicle membrane that affects the proton electrochemical gradient and promotes synaptic vesicles acidification. Moreover, may function as a K(+)/H(+) antiport allowing to maintain the electrical gradient and to decrease chemical gradient and therefore sustain vesicular glutamate uptake. The vesicular K(+)/H(+) antiport activity is electroneutral. At the plasma membrane, following exocytosis, functions as a symporter of Na(+) and phosphate from the extracellular space to the cytoplasm allowing synaptic phosphate homeostasis regulation. The symporter activity is driven by an inside negative membrane potential and is electrogenic. Is necessary for synaptic signaling of visual-evoked responses from photoreceptors.
Protein Name Vesicular Glutamate Transporter 1
Vglut1
Brain-Specific Na(+-Dependent Inorganic Phosphate Cotransporter
Solute Carrier Family 17 Member 7
Database Links Reactome: R-HSA-210500
Reactome: R-HSA-428643
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Cell Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Alternative Antibody Names Anti-Vesicular Glutamate Transporter 1 antibody
Anti-Vglut1 antibody
Anti-Brain-Specific Na(+-Dependent Inorganic Phosphate Cotransporter antibody
Anti-Solute Carrier Family 17 Member 7 antibody
Anti-SLC17A7 antibody
Anti-BNPI antibody
Anti-VGLUT1 antibody

Information sourced from Uniprot.org

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