• Western blot analysis of extracts of various cell lines, using SLC12A2 antibody (STJ113275) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using SLC12A2 rabbit polyclonal antibody (STJ113275) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using SLC12A2 rabbit polyclonal antibody (STJ113275) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-SLC12A2 antibody (120-1212) (STJ113275)

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STJ113275

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SLC12A2 (120-1212) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SLC12A2
Gene ID: 6558
Uniprot ID: S12A2_HUMAN
Immunogen Region: 120-1212
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 120-1212 of human SLC12A2 (NP_001037.1).
Immunogen Sequence: GEASGESEPAKGSEEAKGRF RVNFVDPAASSSAEDSLSDA AGVGVDGPNVSFQNGGDTVL SEGSSLHSGGGGGSGHHQHY YYDTHTNTYYLRTFGHNTMD AVPRIDHYRHTAAQLGEKLL RPSLAELHDELEKEPFEDGF ANGEESTPTRDAVVTYTAES K
Tissue Specificity Expressed in many tissues.
Post Translational Modifications Phosphorylated at Thr-203, Thr-207 and Thr-212 by OXSR1/OSR1 and STK39/SPAK downstream of WNK kinases (WNK1, WNK2, WNK3 or WNK4), promoting its activity.
Function Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume.
Protein Name Solute Carrier Family 12 Member 2
Basolateral Na-K-Cl Symporter
Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2
Database Links Reactome: R-HSA-426117
Cellular Localisation Basolateral Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Solute Carrier Family 12 Member 2 antibody
Anti-Basolateral Na-K-Cl Symporter antibody
Anti-Bumetanide-Sensitive Sodium-(Potassium-Chloride Cotransporter 2 antibody
Anti-SLC12A2 antibody
Anti-NKCC1 antibody

Information sourced from Uniprot.org

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