| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Nad-Dependent Protein Deacetylase Sirtuin-3-Mitochondrial (300-399) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S4834RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIRT3 |
| Gene ID: |
23410 |
| Uniprot ID: |
SIR3_HUMAN |
| Immunogen Region: |
300-399 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 300-399 of human SIRT3 (NP_036371.1). |
| Immunogen Sequence: |
QRFLLHVVDFPMADLLLILG TSLEVEPFASLTEAVRSSVP RLLINRDLVGPLAWHPRSRD VAQLGDVVHGVESLVELLGW TEEMRDLVQRETGKLDGPDK |
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. |
| Function | NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation. Regulates hepatic lipogenesis. Uses NAD(+) substrate imported by SLC25A47, triggering downstream activation of PRKAA1/AMPK-alpha signaling cascade that ultimately downregulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. |
| Protein Name | Nad-Dependent Protein Deacetylase Sirtuin-3 - MitochondrialHsirt3Regulatory Protein Sir2 Homolog 3Sir2-Like Protein 3 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-9615017Reactome: R-HSA-9617629 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Deacetylase Sirtuin-3 - Mitochondrial antibodyAnti-Hsirt3 antibodyAnti-Regulatory Protein Sir2 Homolog 3 antibodyAnti-Sir2-Like Protein 3 antibodyAnti-SIRT3 antibodyAnti-SIR2L3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
