| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SIGLEC8 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literatu |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SIGLEC8 |
| Gene ID: | 27181 |
| Uniprot ID: | SIGL8_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_055257.2 |
| Immunogen Sequence: | SYKSQLNYKTKQ |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes. |
| Protein Name | Sialic Acid-Binding Ig-Like Lectin 8Siglec-8Sialoadhesin Family Member 2Saf-2 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Sialic Acid-Binding Ig-Like Lectin 8 antibodyAnti-Siglec-8 antibodyAnti-Sialoadhesin Family Member 2 antibodyAnti-Saf-2 antibodyAnti-SIGLEC8 antibodyAnti-SAF2 antibody |
Information sourced from Uniprot.org