| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SIGLEC8 (17-363) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SIGLEC8 |
| Gene ID: |
27181 |
| Uniprot ID: |
SIGL8_HUMAN |
| Immunogen Region: |
17-363 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human Siglec-8 (NP_055257.2). |
| Immunogen Sequence: |
MEGDRQYGDGYLLQVQELVT VQEGLCVHVPCSFSYPQDGW TDSDPVHGYWFRAGDRPYQD APVATNNPDREVQAETQGRF QLLGDIWSNDCSLSIRDARK RDKGSYFFRLERGSMKWSYK SQLNYKTKQLSVFVTALTHR PDILILGTLESGHSRNLTCS VPWACKQGTPPMISWIGASV SSPGPTTARSSVLTLTPKPQ DHGTSLTCQVTLPGTGVTTT STVRLDVSYPPWNLTMTVF |
| Tissue Specificity | Expressed specifically on red blood cells namely basophil, mast cells and eosinophils. |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes. |
| Protein Name | Sialic Acid-Binding Ig-Like Lectin 8Siglec-8Sialoadhesin Family Member 2Saf-2 |
| Database Links | Reactome: R-HSA-198933 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Sialic Acid-Binding Ig-Like Lectin 8 antibodyAnti-Siglec-8 antibodyAnti-Sialoadhesin Family Member 2 antibodyAnti-Saf-2 antibodyAnti-SIGLEC8 antibodyAnti-SAF2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89075/357532/STJ11100002_1__24167.1713121647.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Adenosine Deaminase (Adenosine Deaminase (ADA) ) knockout (KO) HeLa cells, using [KO Validated] Adenosine Deaminase (ADA) Rabbit polyclonal antibody (STJ11100002) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")