• Confocal imaging of NIH/3T3 cells using SFRP4 Rabbit monoclonal antibody (STJ11102014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using SFRP4 Rabbit monoclonal antibody (STJ11102014, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of SFRP4 in paraffin-embedded human colon using SFRP4 Rabbit monoclonal antibody (STJ11102014) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using SFRP4 Rabbit monoclonal antibody (STJ11102014) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SFRP4 antibody (201-300) [S4MR] (STJ11102014)

SKU:
STJ11102014

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SFRP4 (201-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SFRP4
Gene ID: 6424
Uniprot ID: SFRP4_HUMAN
Immunogen Region: 201-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 201-300 of human SFRP4 (Q6FHJ7).
Immunogen Sequence: AKIKAVQRSGCNEVTTVVDV KEIFKSSSPIPRTQVPLITN SSCQCPHILPHQDVLIMCYE WRSRMMLLENCLVEKWRDQL SKRSIQWEERLQEQRRTVQD
Tissue Specificity Expressed in mesenchymal cells. Highly expressed in the stroma of proliferative endometrium. Expressed in cardiomyocytes. Shows moderate to strong expression in ovarian tumors with expression increasing as the tumor stage increases. In ovarian tumors, expression levels are inversely correlated with expression of CTNNB1 (at protein level).
Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake.
Protein Name Secreted Frizzled-Related Protein 4
Sfrp-4
Frizzled Protein - Human Endometrium
Frphe
Cellular Localisation Secreted
Cytoplasmic In Ovarian Tumor Cells
Alternative Antibody Names Anti-Secreted Frizzled-Related Protein 4 antibody
Anti-Sfrp-4 antibody
Anti-Frizzled Protein - Human Endometrium antibody
Anti-Frphe antibody
Anti-SFRP4 antibody
Anti-FRPHE antibody

Information sourced from Uniprot.org

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