• Western blot analysis of extracts of HepG2 cells, using SF1 antibody (STJ28507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SF1 antibody (1-280) (STJ28507)

SKU:
STJ28507

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SF1 (1-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SF1
Gene ID: 7536
Uniprot ID: SF01_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1).
Immunogen Sequence: MATGANATPLDFPSKKRKRS RWNQDTMEQKTVIPGMPTVI PPGLTREQERAYIVQLQIED LTRKLRTGDLGIPPNPEDRS PSPEPIYNSEGKRLNTREFR TRKKLEEERHNLITEMVALN PDFKPPADYKPPATRVSDKV MIPQDEYPEINFVGLLIGPR GNTLKNIEKECNAKIMIRGK GSVKEGKVGRKDGQMLPGED EPLHALVTANTMENVKKAVE QIRNILKQGIETPEDQNDL
Tissue Specificity Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Post Translational Modifications Phosphorylation on Ser-20 interferes with U2AF2 binding and spliceosome assembly. Isoform 6 is phosphorylated on Ser-463.
Function Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Protein Name Splicing Factor 1
Mammalian Branch Point-Binding Protein
Bbp
Mbbp
Transcription Factor Zfm1
Zinc Finger Gene In Men1 Locus
Zinc Finger Protein 162
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Splicing Factor 1 antibody
Anti-Mammalian Branch Point-Binding Protein antibody
Anti-Bbp antibody
Anti-Mbbp antibody
Anti-Transcription Factor Zfm1 antibody
Anti-Zinc Finger Gene In Men1 Locus antibody
Anti-Zinc Finger Protein 162 antibody
Anti-SF1 antibody
Anti-ZFM1 antibody
Anti-ZNF162 antibody

Information sourced from Uniprot.org

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