Western blot analysis of extracts of various cell lines, using SETDB2 antibody (STJ29528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SETDB2 antibody (148-378) (STJ29528)

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STJ29528
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SETDB2
Gene ID: 83852
Uniprot ID: SETB2_HUMAN
Immunogen Region: 148-378
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 148-378 of human SETDB2 (NP_114121.2).
Immunogen Sequence: SGACLMKMPLNLKGENPLQL PIKCHFQRRHAKTNSHSSAL HVSYKTPCGRSLRNVEEVFR YLLETECNFLFTDNFSFNTY VQLARNYPKQKEVVSDVDIS NGVESVPISFCNEIDSRKLP QFKYRKTVWPRAYNLTNFSS MFTDSCDCSEGCIDITKCAC LQLTARNAKTSPLSSDKITT GYKYKRLQRQIPTGIYECSL LCKCNRQLCQNRVVQHGPQV RLQVFKTEQKG
Tissue Specificity Ubiquitous. Highest expression in heart, testis and ovary.
Function Histone methyltransferase involved in left-right axis specification in early development and mitosis. Specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). H3K9me3 is a specific tag for epigenetic transcriptional repression that recruits HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Contributes to H3K9me3 in both the interspersed repetitive elements and centromere-associated repeats. Plays a role in chromosome condensation and segregation during mitosis.
Protein Name Histone-Lysine N-Methyltransferase Setdb2
Chronic Lymphocytic Leukemia Deletion Region Gene 8 Protein
Lysine N-Methyltransferase 1f
Set Domain Bifurcated 2
Database Links Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Setdb2 antibody
Anti-Chronic Lymphocytic Leukemia Deletion Region Gene 8 Protein antibody
Anti-Lysine N-Methyltransferase 1f antibody
Anti-Set Domain Bifurcated 2 antibody
Anti-SETDB2 antibody
Anti-C13orf4 antibody
Anti-CLLD8 antibody
Anti-KMT1F antibody

Information sourced from Uniprot.org

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