| Host: |
Mouse |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Monkey |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Histone-lysine N-methyltransferase SETDB1 is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
5H6D4 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SETDB1 |
| Gene ID: |
9869 |
| Uniprot ID: |
SETB1_HUMAN |
| Specificity: |
ESET Monoclonal Antibody detects endogenous levels of ESET protein. |
| Immunogen: |
Purified recombinant fragment of human ESET expressed in E. Coli. |
| Post Translational Modifications | Degraded by the proteasome, shielded by interaction with ATF7IP. |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway. Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions. |
| Protein Name | Histone-Lysine N-Methyltransferase Setdb1Erg-Associated Protein With Set DomainEsetHistone H3-K9 Methyltransferase 4H3-K9-Hmtase 4Lysine N-Methyltransferase 1eSet Domain Bifurcated 1 |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusCytoplasmChromosomeAssociated With Non-Pericentromeric Regions Of ChromatinExcluded From Nucleoli And Islands Of Condensed Chromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setdb1 antibodyAnti-Erg-Associated Protein With Set Domain antibodyAnti-Eset antibodyAnti-Histone H3-K9 Methyltransferase 4 antibodyAnti-H3-K9-Hmtase 4 antibodyAnti-Lysine N-Methyltransferase 1e antibodyAnti-Set Domain Bifurcated 1 antibodyAnti-SETDB1 antibodyAnti-ESET antibodyAnti-KIAA0067 antibodyAnti-KMT1E antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
