• Western blot analysis of extracts of various cell lines, using SET/TAF1 rabbit monoclonal antibody (STJ11102445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SET/TAF1 antibody (STJ11102445). Western blot was performed from the immunoprecipitate using SET/TAF1 antibody (STJ11102445) at a dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded rat brain using SET/TAF1 rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of U-2 OS cells using SET/TAF1 Rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using SET/TAF1 rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SET/TAF1 in paraffin-embedded mouse spleen using SET/TAF1 Rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse spleen using SET/TAF1 rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SET/TAF1 in paraffin-embedded human esophageal cancer using SET/TAF1 Rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using SET/TAF1 rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SET/TAF1 in paraffin-embedded rat brain using SET/TAF1 Rabbit monoclonal antibody (STJ11102445) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SET/TAF1 antibody (STJ11102445). Western blot was performed from the immunoprecipitate using SET/TAF1 antibody (STJ11102445) at a dilition of 1:1000.
  • Western blot analysis of various lysates using SET/TAF1 Rabbit monoclonal antibody (STJ11102445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SET antibody (200-290) [S5MR] (STJ11102445)

SKU:
STJ11102445

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SET (200-290) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SET
Gene ID: 6418
Uniprot ID: SET_HUMAN
Immunogen Region: 200-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-290 of human SET/TAF1 (Q01105).
Immunogen Sequence: ESFFTWFTDHSDAGADELGE VIKDDIWPNPLQYYLVPDMD DEEGEGEEDDDDDEEEEGLE DIDEEGDEDEGEEDEDDDEG EEGEEDEGEDD
Tissue Specificity Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.
Post Translational Modifications Isoform 2 is phosphorylated on Ser-15 and Ser-24. Isoform 2 is acetylated on Lys-11. Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated. Isoform 2: Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1.
Function Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins.however, isoform 2 specific activity is higher.
Protein Name Protein Set
Hla-Dr-Associated Protein Ii
Inhibitor Of Granzyme A-Activated Dnase
Igaad
Phapii
Phosphatase 2a Inhibitor I2pp2a
I-2pp2a
Template-Activating Factor I
Taf-I
Database Links Reactome: R-HSA-2299718
Reactome: R-HSA-450520
Cellular Localisation Cytoplasm
Cytosol
Endoplasmic Reticulum
Nucleus
Nucleoplasm
In The Cytoplasm
Found Both In The Cytosol And Associated With The Endoplasmic Reticulum
The Set Complex Is Associated With The Endoplasmic Reticulum
Following Ctl Attack And Cleavage By Gzma
Moves Rapidly To The Nucleus
Where It Is Found In The Nucleoplasm
Avoiding The Nucleolus
Similar Translocation To The Nucleus Is Also Observed For Lymphocyte-Activated Killer Cells After The Addition Of Calcium
Alternative Antibody Names Anti-Protein Set antibody
Anti-Hla-Dr-Associated Protein Ii antibody
Anti-Inhibitor Of Granzyme A-Activated Dnase antibody
Anti-Igaad antibody
Anti-Phapii antibody
Anti-Phosphatase 2a Inhibitor I2pp2a antibody
Anti-I-2pp2a antibody
Anti-Template-Activating Factor I antibody
Anti-Taf-I antibody
Anti-SET antibody

Information sourced from Uniprot.org

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