• Immunohistochemistry analysis of sestrin 1 in paraffin-embedded mouse heart using sestrin 1 Rabbit monoclonal antibody (STJ11103135) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of sestrin 1 in paraffin-embedded human colon carcinoma using sestrin 1 Rabbit monoclonal antibody (STJ11103135) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using sestrin 1 Rabbit monoclonal antibody (STJ11103135) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-sestrin 1 antibody (100-200) [S5MR] (STJ11103135)

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STJ11103135

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-sestrin 1 (100-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SESN1
Gene ID: 27244
Uniprot ID: SESN1_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human sestrin 1 (Q9Y6P5).
Immunogen Sequence: KTQHYLLQMDGPLPLHYRHY IGIMAAARHQCSYLVNLHVN DFLHVGGDPKWLNGLENAPQ KLQNLGELNKVLAHRPWLIT KEHIEGLLKAEEHSWSLAEL V
Tissue Specificity Widely expressed.
Function Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses. May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. Moreover, may prevent the accumulation of reactive oxygen species (ROS) through the alkylhydroperoxide reductase activity born by the N-terminal domain of the protein. Was originally reported to contribute to oxidative stress resistance by reducing PRDX1. However, this could not be confirmed.
Protein Name Sestrin-1
P53-Regulated Protein Pa26
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-9639288
Reactome: R-HSA-9755511
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Sestrin-1 antibody
Anti-P53-Regulated Protein Pa26 antibody
Anti-SESN1 antibody
Anti-PA26 antibody
Anti-SEST1 antibody

Information sourced from Uniprot.org

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