• Confocal imaging of NIH/3T3 cells using HSP47/SERPINH1 Rabbit monoclonal antibody (STJ11101393, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of U-2 OS cells using HSP47/SERPINH1 Rabbit monoclonal antibody (STJ11101393, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of HSP47/SERPINH1 in paraffin-embedded human colon using HSP47/SERPINH1 Rabbit monoclonal antibody (STJ11101393) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using HSP47/SERPINH1 Rabbit monoclonal antibody (STJ11101393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SERPINH1 antibody (319-418) [S3MR] (STJ11101393)

SKU:
STJ11101393

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Hsp47 (319-418) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINH1
Gene ID: 871
Uniprot ID: SERPH_HUMAN
Immunogen Region: 319-418
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 319-418 of human HSP47/SERPINH1 (P50454).
Immunogen Sequence: KHLAGLGLTEAIDKNKADLS RMSGKKDLYLASVFHATAFE LDTDGNPFDQDIYGREELRS PKLFYADHPFIFLVRDTQSG SLLFIGRLVRPKGDKMRDEL
Function Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Protein Name Serpin H1
47 Kda Heat Shock Protein
Arsenic-Transactivated Protein 3
Astp3
Cell Proliferation-Inducing Gene 14 Protein
Collagen-Binding Protein
Colligin
Rheumatoid Arthritis-Related Antigen Ra-A47
Database Links Reactome: R-HSA-1650814
Cellular Localisation Endoplasmic Reticulum Lumen
Alternative Antibody Names Anti-Serpin H1 antibody
Anti-47 Kda Heat Shock Protein antibody
Anti-Arsenic-Transactivated Protein 3 antibody
Anti-Astp3 antibody
Anti-Cell Proliferation-Inducing Gene 14 Protein antibody
Anti-Collagen-Binding Protein antibody
Anti-Colligin antibody
Anti-Rheumatoid Arthritis-Related Antigen Ra-A47 antibody
Anti-SERPINH1 antibody
Anti-CBP1 antibody
Anti-CBP2 antibody
Anti-HSP47 antibody
Anti-SERPINH2 antibody
Anti-PIG14 antibody

Information sourced from Uniprot.org

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