• Immunofluorescence analysis of HepG2 cells using SSCA1/SerpinB3 Rabbit polyclonal antibody (STJ27371) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A431 cells using SSCA1/SerpinB3 Rabbit polyclonal antibody (STJ27371) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SSCA1/SerpinB3 using SSCA1/SerpinB3 Rabbit polyclonal antibody (STJ27371) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SERPINB3 antibody (2-390) (STJ27371)

SKU:
STJ27371

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINB3 (2-390) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINB3
Gene ID: 6317
Uniprot ID: SPB3_HUMAN
Immunogen Region: 2-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-390 of human SSCA1/SerpinB3 (NP_008850.1).
Immunogen Sequence: NSLSEANTKFMFDLFQQFRK SKENNIFYSPISITSALGMV LLGAKDNTAQQIKKVLHFDQ VTENTTGKAATYHVDRSGNV HHQFQKLLTEFNKSTDAYEL KIANKLFGEKTYLFLQEYLD AIKKFYQTSVESVDFANAPE ESRKKINSWVESQTNEKIKN LIPEGNIGSNTTLVLVNAIY FKGQWEKKFNKEDTKEEKFW PNKNTYKSIQMMRQYTSFHF ASLEDVQAKVLEIPYKGKD
Tissue Specificity Squamous cells. Expressed in some hepatocellular carcinoma (at protein level).
Function May act as a papain-like cysteine protease inhibitor to modulate the host immune response against tumor cells. Also functions as an inhibitor of UV-induced apoptosis via suppression of the activity of c-Jun NH(2)-terminal kinase (JNK1).
Protein Name Serpin B3
Protein T4-A
Squamous Cell Carcinoma Antigen 1
Scca-1
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Seems To Also Be Secreted In Plasma By Cancerous Cells But At A Low Level
Alternative Antibody Names Anti-Serpin B3 antibody
Anti-Protein T4-A antibody
Anti-Squamous Cell Carcinoma Antigen 1 antibody
Anti-Scca-1 antibody
Anti-SERPINB3 antibody
Anti-SCCA antibody
Anti-SCCA1 antibody

Information sourced from Uniprot.org

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