• Western blot analysis of various lysates, using Alpha-1 Antichymotrypsin (SERPINA3) Rabbit polyclonal antibody (STJ26163) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SERPINA3 antibody (24-423) (STJ26163)

SKU:
STJ26163

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SERPINA3 (24-423) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SERPINA3
Gene ID: 12
Uniprot ID: AACT_HUMAN
Immunogen Region: 24-423
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 24-423 of human Alpha-1 Antichymotrypsin (Alpha-1 Antichymotrypsin (SERPINA3) ) (NP_001076.2).
Immunogen Sequence: HPNSPLDEENLTQENQDRGT HVDLGLASANVDFAFSLYKQ LVLKAPDKNVIFSPLSISTA LAFLSLGAHNTTLTEILKGL KFNLTETSEAEIHQSFQHLL RTLNQSSDELQLSMGNAMFV KEQLSLLDRFTEDAKRLYGS EAFATDFQDSAAAKKLINDY VKNGTRGKITDLIKDLDSQT MMVLVNYIFFKAKWEMPFDP QDTHQSRFYLSKKKWVMVPM MSLHHLTIPYFRDEELSCT
Tissue Specificity Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
Post Translational Modifications N- and O-glycosylated.
Function Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
Protein Name Alpha-1-Antichymotrypsin
Act
Cell Growth-Inhibiting Gene 24/25 Protein
Serpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-6798695
Cellular Localisation Secreted
Alternative Antibody Names Anti-Alpha-1-Antichymotrypsin antibody
Anti-Act antibody
Anti-Cell Growth-Inhibiting Gene 24/25 Protein antibody
Anti-Serpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less antibody
Anti-SERPINA3 antibody
Anti-AACT antibody
Anti-GIG24 antibody
Anti-GIG25 antibody

Information sourced from Uniprot.org

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