• Immunohistochemistry analysis of SENP1 in paraffin-embedded Human liver cancer using SENP1 Rabbit polyclonal antibody (STJ115053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SENP1 in paraffin-embedded Rat testis using SENP1 Rabbit polyclonal antibody (STJ115053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SENP1 in paraffin-embedded Mouse testis using SENP1 Rabbit polyclonal antibody (STJ115053) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SENP1 Rabbit polyclonal antibody (STJ115053) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SENP1 antibody (220-420) (STJ115053)

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STJ115053

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SENP1 (220-420) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SENP1
Gene ID: 29843
Uniprot ID: SENP1_HUMAN
Immunogen Region: 220-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 220-420 of human SENP1 (NP_001254523.1).
Immunogen Sequence: SRCLSSSKNTLKDSLFKNGN SCASQIIGSDTSSSGSASIL TNQEQLSHSVYSLSSYTPDV AFGSKDSGTLHHPHHHHSVP HQPDNLAASNTQSEGSDSVI LLKVKDSQTPTPSSTFFQAE LWIKELTSVYDSRARERLRQ IEEQKALALQLQNQRLQERE HSVHDSVELHLRVPLEKEIP VTVVQETQKKGHKLTDSEDE F
Tissue Specificity Highly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
Function Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1 and BHLHE40/DEC1, which decreases its transcriptional repression activity. Deconjugates SUMO1 from CLOCK, which decreases its transcriptional activation activity. Deconjugates SUMO2 from MTA1. Deconjugates SUMO1 from METTL3. Desumoylates CCAR2 which decreases its interaction with SIRT1. Deconjugates SUMO1 from GPS2.
Protein Name Sentrin-Specific Protease 1
Sentrin/Sumo-Specific Protease Senp1
Database Links Reactome: R-HSA-3065679
Reactome: R-HSA-9035034
Cellular Localisation Nucleus
Cytoplasm
Shuttles Between Cytoplasm And Nucleus
Alternative Antibody Names Anti-Sentrin-Specific Protease 1 antibody
Anti-Sentrin/Sumo-Specific Protease Senp1 antibody
Anti-SENP1 antibody

Information sourced from Uniprot.org

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