• Immunohistochemistry analysis of paraffin-embedded human stomach using CD62P/P-selectin antibody (STJ29851) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using CD62P/P-selectin antibody (STJ29851) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using CD62P/P-selectin antibody (STJ29851) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CD62P/P-selectin antibody (STJ29851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Anti-SELP antibody (30-200) (STJ29851)

SKU:
STJ29851

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SELP (30-200) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SELP
Gene ID: 6403
Uniprot ID: LYAM3_HUMAN
Immunogen Region: 30-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human CD62P/P-selectin (NP_002996.2).
Immunogen Sequence: ISELTNQKEVAAWTYHYSTK AYSWNISRKYCQNRYTDLVA IQNKNEIDYLNKVLPYYSSY YWIGIRKNNKTWTWVGTKKA LTNEAENWADNEPNNKRNNE DCVEIYIKSPSAPGKWNDEH CLKKKHALCYTASCQDMSCS KQGECLETIGNYTCSCYPGF YGPECEYVREC
Tissue Specificity Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Function Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
Protein Name P-Selectin
Cd62 Antigen-Like Family Member P
Granule Membrane Protein 140
Gmp-140
Leukocyte-Endothelial Cell Adhesion Molecule 3
Lecam3
Platelet Activation Dependent Granule-External Membrane Protein
Padgem
Cd Antigen Cd62p
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-P-Selectin antibody
Anti-Cd62 Antigen-Like Family Member P antibody
Anti-Granule Membrane Protein 140 antibody
Anti-Gmp-140 antibody
Anti-Leukocyte-Endothelial Cell Adhesion Molecule 3 antibody
Anti-Lecam3 antibody
Anti-Platelet Activation Dependent Granule-External Membrane Protein antibody
Anti-Padgem antibody
Anti-Cd Antigen Cd62p antibody
Anti-SELP antibody
Anti-GMRP antibody
Anti-GRMP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance