Anti-SELENOK antibody (1-94) (STJ11103383)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SELENOK (1-94) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SELENOK |
| Gene ID | 58515 |
| Uniprot ID | SELK_HUMAN |
| Immunogen Region | 1-94 |
| Immunogen Sequence | MVYISNGQVLDSRSQSPWRL SLITDFFWGIAEFVVLFFKT LLQQDVKKRRSYGNSSDSRY DDGRGPPGNPPRRMGRINHL RGPSPPPMAGGUGR |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-94 of human SELENOK (NP_067060.2). |
Additional Info
| Tissue Specificity | Highly expressed in heart. |
| Post Translational Modifications | Cleaved by CAPN2/m-calpain in resting macrophages but not in activated macrophages. Macrophage activation up-regulates expression of the calpain inhibitor CAST/calpastatin, resulting in inhibition of CAPN2 activity. Truncated SELENOK proteins produced by failed UGA/Sec decoding are ubiquitinated by the CRL2(KLHDC2) complex, which recognizes the diglycine (Gly-Gly) at the C-terminus of truncated SELENOK proteins. |
| Function | Required for Ca(2+) flux in immune cells and plays a role in T-cell proliferation and in T-cell and neutrophil migration. Involved in endoplasmic reticulum-associated degradation (ERAD) of soluble glycosylated proteins. Required for palmitoylation and cell surface expression of CD36 and involved in macrophage uptake of low-density lipoprotein and in foam cell formation. Together with ZDHHC6, required for palmitoylation of ITPR1 in immune cells, leading to regulate ITPR1 stability and function. Plays a role in protection of cells from ER stress-induced apoptosis. Protects cells from oxidative stress when overexpressed in cardiomyocytes. |
| Protein Name | Selenoprotein KSelk |
| Database Links | |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinCell MembraneProbably Mainly Localized In The Er |
| Alternative Antibody Names | Anti-Selenoprotein K antibodyAnti-Selk antibodyAnti-SELENOK antibodyAnti-SELK antibodyAnti-HSPC030 antibodyAnti-HSPC297 antibody |
Information sourced from Uniprot.org