• Confocal imaging of HeLa cells using SDHA Rabbit monoclonal antibody (STJ11101431, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Confocal imaging of paraffin-embedded Human colon cancer tissue using SDHA Rabbit monoclonal antibody (STJ11101431, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using SDHA Rabbit monoclonal antibody (STJ11101431) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SDHA antibody (550-650) [S1MR] (STJ11101431)

SKU:
STJ11101431

£146.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-SDHA (550-650) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SDHA
Gene ID: 6389
Uniprot ID: SDHA_HUMAN
Immunogen Region: 550-650
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 550-650 of human SDHA (P31040).
Immunogen Sequence: KTFDRGMVWNTDLVETLELQ NLMLCALQTIYGAEARKESR GAHAREDYKVRIDEYDYSKP IQGQQKKPFEEHWRKHTLSY VDVGTGKVTLEYRPVIDKTL N
Post Translational Modifications Phosphorylation at Tyr-215 is important for efficient electron transfer in complex II and the prevention of ROS generation. Acetylated. Deacetylated by SIRT3.
Function Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor.
Protein Name Succinate Dehydrogenase Ubiquinone Flavoprotein Subunit - Mitochondrial
Flavoprotein Subunit Of Complex Ii
Fp
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Succinate Dehydrogenase Ubiquinone Flavoprotein Subunit - Mitochondrial antibody
Anti-Flavoprotein Subunit Of Complex Ii antibody
Anti-Fp antibody
Anti-SDHA antibody
Anti-SDH2 antibody
Anti-SDHF antibody

Information sourced from Uniprot.org

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