| Host: | Goat |
| Applications: | Pep-ELISA/WB/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-SDCCAG8 (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000. WB: Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 82.7kDa according to NP_006633.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | SDCCAG8 |
| Gene ID: | 10806 |
| Uniprot ID: | SDCG8_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_006633.1 |
| Immunogen Sequence: | TINDQSQYIHHLEAE |
| Function | Plays a role in the establishment of cell polarity and epithelial lumen formation. Also plays an essential role in ciliogenesis and subsequent Hedgehog signaling pathway that requires the presence of intact primary cilia for pathway activation. Mechanistically, interacts with and mediates RABEP2 centrosomal localization which is critical for ciliogenesis. |
| Protein Name | Serologically Defined Colon Cancer Antigen 8 Antigen Ny-Co-8 Centrosomal Colon Cancer Autoantigen Protein Hcccap |
| Database Links | Reactome: R-HSA-2565942 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-5620912 Reactome: R-HSA-8854518 |
| Cellular Localisation | Cytoplasm Cytoskeleton Microtubule Organizing Center Centrosome Centriole Cilium Basal Body Cell Junction Located At The Distal Ends Of Both Centrioles And Colocalizes To Centrosomes Throughout The Cell Cycle Isoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Serologically Defined Colon Cancer Antigen 8 antibody Anti-Antigen Ny-Co-8 antibody Anti-Centrosomal Colon Cancer Autoantigen Protein antibody Anti-Hcccap antibody Anti-SDCCAG8 antibody Anti-CCCAP antibody Anti-NPHP10 antibody Anti-HSPC085 antibody |
Information sourced from Uniprot.org