• Immunohistochemistry analysis of paraffin-embedded mouse lung using SCNN1G antibody (STJ27879) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using SCNN1G antibody (STJ27879) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 60s.

Anti-SCNN1G antibody (85-355) (STJ27879)

SKU:
STJ27879

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SCNN1G (85-355) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SCNN1G
Gene ID: 6340
Uniprot ID: SCNNG_HUMAN
Immunogen Region: 85-355
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 85-355 of human SCNN1G (NP_001030.2).
Immunogen Sequence: IKVHFRKLDFPAVTICNINP YKYSTVRHLLADLEQETREA LKSLYGFPESRKRREAESWN SVSEGKQPRFSHRIPLLIFD QDEKGKARDFFTGRKRKVGG SIIHKASNVMHIESKQVVGF QLCSNDTSDCATYTFSSGIN AIQEWYKLHYMNIMAQVPLE KKINMSYSAEELLVTCFFDG VSCDARNFTLFHHPMHGNCY TFNNRENETILSTSMGGSEY GLQVILYINEEEYNPFLVS
Tissue Specificity Expressed in kidney (at protein level).
Post Translational Modifications Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation. Ubiquitinated.this targets individual subunits for endocytosis and proteasome-mediated degradation. ENaC cleavage by furin, and subsequently by prostasin (PRSS8), leads to a stepwise increase in the open probability of the channel as a result of release of the alpha and gamma subunit inhibitory tracts, respectively. Interaction of ENaC subunit SCNN1B with BPIFA1 protects ENaC against proteolytic activation.
Function Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
Protein Name Amiloride-Sensitive Sodium Channel Subunit Gamma
Epithelial Na(+ Channel Subunit Gamma
Enacg
Gamma-Enac
Gamma-Nach
Nonvoltage-Gated Sodium Channel 1 Subunit Gamma
Scneg
Database Links Reactome: R-HSA-2672351
Reactome: R-HSA-9730628
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Apical Membrane Of Epithelial Cells
Alternative Antibody Names Anti-Amiloride-Sensitive Sodium Channel Subunit Gamma antibody
Anti-Epithelial Na(+ Channel Subunit Gamma antibody
Anti-Enacg antibody
Anti-Gamma-Enac antibody
Anti-Gamma-Nach antibody
Anti-Nonvoltage-Gated Sodium Channel 1 Subunit Gamma antibody
Anti-Scneg antibody
Anti-SCNN1G antibody

Information sourced from Uniprot.org

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