• Western blot analysis of extracts of normal 293T cells and 293T transfected with Spike S1 Protein, using SARS-CoV-2 Spike S1 antibody (STJ11101793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
  • Immobilized Recombinant SARS-COV-2 Spike S1 Protein (STJP000023LQ) at 1μg/mL (100μL/well) can bind SARS-CoV-2 Spike S1 Rabbit pAb (STJ11101793) with a linear range of 0. 78-50ng/mL.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike S1 fusion protein (top left) and untreated 293T cells (top right) use SARS-CoV-2 Spike S1 Rabbit pAb (STJ11101793) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike S1 antibody (STJ11101793). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike S1 antibody (STJ11101793) at a dilition of 1:1000.

Anti-SARS-CoV-2 Spike S1-antibody (STJ11101793)

SKU:
STJ11101793

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Host: Rabbit
Applications: WB, IF, IP
Reactivity: SARS-CoV-2
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2 Spike S1 is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:20000-1:80000WB 1:500-1:2000
IF 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Immunogen: Recombinant fusion protein of SARS-CoV-2 Spike S1.
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance