• Western blot analysis of extracts of 293T cells, using SARS-CoV-2 Spike antibody (STJ11101794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of 293T cells, using SARS-CoV-2 Spike antibody (STJ11101794) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immobilized Recombinant SARS-COV-2 S1+S2 ECD (S-ECD) Protein (RP01283) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Spike rabbit polyclonal antibody (STJ11101794) with a linear range of 0. 78-50ng/mL.
  • Immunofluorescence analysis of 293T cells transfected with SARS-CoV-2 Spike protein and untreated 293T cells use SARS-CoV-2 Spike rabbit polyclonal antibody (STJ11101794) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug SARS-CoV-2 Spike antibody (STJ11101794). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Spike antibody (STJ11101794) at a dilition of 1:1000.

Anti-SARS-CoV-2 Spike antibody (STJ11101794)

SKU:
STJ11101794

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Host: Rabbit
Applications: WB, IF, IP
Reactivity: SARS-CoV-2
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-SARS-CoV-2 Spike is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: ELISA 1:40000-1:160000
WB 1:500-1:2000
IF 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Immunogen: A synthetic peptide of SARS-CoV-2 Spike.
Background The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It NA NA s been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. S1 mainly contains a receptor binding domain (RBD) , which is responsible for recognizing the cell surface receptor. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD) , leading to a higher mortality rate.

Information sourced from Uniprot.org

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