Western blot analysis of lysates from control 293T and SARS-CoV-2 Spike S1-293T trasfected cells, using SARS-CoV-2 Spike S1 mAb, BSA and glycerol free (STJ11101211) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-2019-nCoV antibody [S1MR] (STJ11101211)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11101211
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General Information

Short DescriptionRabbit monoclonal SARS-COV-2 Spike antibody for use in WB, IF, ICC, FC and ELISA in sars-cov-2 samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/FC/ELISA
HostRabbit
ReactivitySARS-CoV-2
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS1MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:2000-1:10000
IF/ICC:1:200-1:800
FC:1:50-1:200
ELISA:1:1000-1:5000
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at 4ยฐC for up to 1 year from the date of receipt. Do not freeze.

Target Information

SpecificityRecombinant fusion protein of SARS-CoV-2 Spike S1.

Additional Info

Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E) , spike or surface glycoprotein (S) , membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Information sourced from Uniprot.org

Citations

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