• Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein, using SARS-CoV-2 Nucleoprotein antibody (STJ11101492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2, using SARS-CoV-2 Nucleoprotein antibody (STJ11101492) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immobilized Recombinant SARS-COV-2 Nucleocapsid (RP01264) at 1 Mu g/mL (100 Mu L/well) can bind SARS-CoV-2 Nucleoprotein Antibody (STJ11101492) with a linear range of 3. 12-200ng/mL.
  • Immunofluorescence analysis of 293T-N and 293T cells using SARS-CoV-2 Nucleoprotein rabbit polyclonal antibody (STJ11101492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Nucleoprotein antibody (STJ11101492). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (STJ11101492) at a dilution of 1:1000.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g SARS-CoV-2 Nucleoprotein antibody (STJ11101492). Western blot was performed from the immunoprecipitate using SARS-CoV-2 Nucleoprotein antibody (STJ11101492) at a dilution of 1:3000.

Anti-SARS-CoV-2 Nucleoprotein antibody (1-420) (STJ11101492)

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STJ11101492

£146.50 - £642.50
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Host: Rabbit
Applications: WB/DB/IF/ICC/IP/ELISA
Reactivity: SARS-CoV-2
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
DB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specifi
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-420
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of coronavirus Nucleoprotein (P0DTC9).
Immunogen Sequence: MSDNGPQNQRNAPRITFGGP SDSTGSNQNGERSGARSKQR RPQGLPNNTASWFTALTQHG KEDLKFPRGQGVPINTNSSP DDQIGYYRRATRRIRGGDGK MKDLSPRWYFYYLGTGPEAG LPYGANKDGIIWVATEGALN TPKDHIGTRNPANNAAIVLQ LPQGTTLPKGFYAEGSRGGS QASSRSSSRSRNSSRNSTPG SSRGTSPARMAGNGGDAALA LLLLDRLNQLESKMSGKGQ
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E) , spike or surface glycoprotein (S) , membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Information sourced from Uniprot.org

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