• Immunofluorescence analysis of U-2 OS cells using SARNP Rabbit polyclonal antibody (STJ115656) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SARNP in paraffin-embedded Human colon using SARNP Rabbit polyclonal antibody (STJ115656) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using SARNP Rabbit polyclonal antibody (STJ115656) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SARNP antibody (1-210) (STJ115656)

SKU:
STJ115656

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SARNP (1-210) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SARNP
Gene ID: 84324
Uniprot ID: SARNP_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human SARNP (NP_149073.1).
Immunogen Sequence: MATETVELHKLKLAELKQEC LARGLETKGIKQDLIHRLQA YLEEHAEEEANEEDVLGDET EEEETKPIELPVKEEEPPEK TVDVAAEKKVVKITSEIPQT ERMQKRAERFNVPVSLESKK AARAARFGISSVPTKGLSSD NKPMVNLDKLKERAQRFGLN VSSISRKSEDDEKLKKRKER FGIVTSSAGTGTTEDTEAKK RKRAERFGIA
Tissue Specificity Low expression in spleen, liver, pancreas, testis, thymus, heart, and kidney. Increased levels are seen in hepatocellular carcinoma and pancreatic adenocarcinoma.
Function Binds both single-stranded and double-stranded DNA with higher affinity for the single-stranded form. Specifically binds to scaffold/matrix attachment region DNA. Also binds single-stranded RNA. Enhances RNA unwinding activity of DDX39A. May participate in important transcriptional or translational control of cell growth, metabolism and carcinogenesis. Component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
Protein Name Sap Domain-Containing Ribonucleoprotein
Cytokine-Induced Protein Of 29 Kda
Nuclear Protein Hcc-1
Proliferation-Associated Cytokine-Inducible Protein Cip29
Database Links Reactome: R-HSA-159236
Reactome: R-HSA-72187
Reactome: R-HSA-73856
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Sap Domain-Containing Ribonucleoprotein antibody
Anti-Cytokine-Induced Protein Of 29 Kda antibody
Anti-Nuclear Protein Hcc-1 antibody
Anti-Proliferation-Associated Cytokine-Inducible Protein Cip29 antibody
Anti-SARNP antibody
Anti-HCC1 antibody
Anti-HSPC316 antibody

Information sourced from Uniprot.org

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