• Immunofluorescence analysis of HUVEC cells, using AOS1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from 293 cells, using AOS1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using SAE1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SAE1 antibody (221-270 aa) (STJ95572)

SKU:
STJ95572

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUMO-activating enzyme subunit 1 (221-270 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SAE1
Gene ID: 10055
Uniprot ID: SAE1_HUMAN
Immunogen Region: 221-270 aa
Specificity: SAE1 Polyclonal Antibody detects endogenous levels of SAE1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AOS1 at the amino acid range 221-270
Function The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Protein Name Sumo-Activating Enzyme Subunit 1
Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed
Database Links Reactome: R-HSA-3065676
Reactome: R-HSA-3065678
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Sumo-Activating Enzyme Subunit 1 antibody
Anti-Ubiquitin-Like 1-Activating Enzyme E1a Cleaved Into - Sumo-Activating Enzyme Subunit 1 - N-Terminally Processed antibody
Anti-SAE1 antibody
Anti-AOS1 antibody
Anti-SUA1 antibody
Anti-UBLE1A antibody

Information sourced from Uniprot.org

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