• Immunohistochemistry analysis of RXRG in paraffin-embedded mouse brain using RXRG Rabbit polyclonal antibody (STJ116161) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RXRG in paraffin-embedded mouse testis using RXRG Rabbit polyclonal antibody (STJ116161) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RXRG in paraffin-embedded rat spleen using RXRG Rabbit polyclonal antibody (STJ116161) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RXRG Rabbit polyclonal antibody (STJ116161) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RXRG antibody (1-137) (STJ116161)

SKU:
STJ116161

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RXRG (1-137) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RXRG
Gene ID: 6258
Uniprot ID: RXRG_HUMAN
Immunogen Region: 1-137
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human RXRG (NP_008848.1).
Immunogen Sequence: MYGNYSHFMKFPAGYGGSPG HTGSTSMSPSAALSTGKPMD SHPSYTDTPVSAPRTLSAVG TPLNALGSPYRVITSAMGPP SGALAAPPGINLVAPPSSQL NVVNSVSSSEDIKPLPGLPG IGNMNYPSTSPGSLVKH
Tissue Specificity Expressed in aortic endothelial cells (at protein level).
Post Translational Modifications Acetylated by EP300.
Function Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid.
Protein Name Retinoic Acid Receptor Rxr-Gamma
Nuclear Receptor Subfamily 2 Group B Member 3
Retinoid X Receptor Gamma
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-5362517
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Retinoic Acid Receptor Rxr-Gamma antibody
Anti-Nuclear Receptor Subfamily 2 Group B Member 3 antibody
Anti-Retinoid X Receptor Gamma antibody
Anti-RXRG antibody
Anti-NR2B3 antibody

Information sourced from Uniprot.org

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