| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RUFY1 |
| Gene ID: |
80230 |
| Uniprot ID: |
RUFY1_HUMAN |
| Immunogen Region: |
439-708 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3). |
| Immunogen Sequence: |
EKDTHEKQDTLVALRQQLEE VKAINLQMFHKAQNAESSLQ QKNEAITSFEGKTNQVMSSM KQMEERLQHSERARQGAEER SHKLQQELGGRIGALQLQLS QLHEQCSSLEKELKSEKEQR QALQRELQHEKDTSSLLRME LQQVEGLKKELRELQDEKAE LQKICEEQEQALQEMGLHLS QSKLKMEDIKEVNQALKGHA WLKDDEATHCRQCEKEFSIS RRKHHCRNCGHIFCNTCSS |
| Tissue Specificity | Broadly expressed, with highest levels in lung, testis, kidney and brain. |
| Post Translational Modifications | Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting. |
| Function | Activating adapter involved in cargo sorting from early/recycling endosomes. Regulates retrieval of proteins from endosomes to the trans-Golgi network through interaction with the dynein-dynactin complex. Dual effector of RAB4B and RAB14, mediates a cooperative interaction allowing endosomal tethering and fusion. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking. In oocytes, self-assembles to form a protein matrix which hold together endolysosomes, autophagosomes and proteasomes and generate non-membrane-bound compartments called endo-lysosomal vesicular assemblies (ELVAs). In immature oocytes, ELVAs sequester ubiquitinated protein aggregates and degrade them upon oocyte maturation. |
| Protein Name | Run And Fyve Domain-Containing Protein 1Fyve-Finger Protein Eip1La-Binding Protein 1Rab4-Interacting ProteinZinc Finger Fyve Domain-Containing Protein 12 |
| Database Links | Reactome: R-HSA-1660499 |
| Cellular Localisation | Early Endosome MembranePeripheral Membrane ProteinIn OocytesLocalizes In Non-Membrane-Bound Compartments Called Endo-Lysosomal Vesicular Assemblies (Elvas) |
| Alternative Antibody Names | Anti-Run And Fyve Domain-Containing Protein 1 antibodyAnti-Fyve-Finger Protein Eip1 antibodyAnti-La-Binding Protein 1 antibodyAnti-Rab4-Interacting Protein antibodyAnti-Zinc Finger Fyve Domain-Containing Protein 12 antibodyAnti-RUFY1 antibodyAnti-RABIP4 antibodyAnti-ZFYVE12 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
