• Western blot analysis of extracts of various cell lines, using RUFY1 antibody (STJ110146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using RUFY1 antibody (STJ110146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-RUFY1 antibody (439-708) (STJ110146)

SKU:
STJ110146

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RUFY1 (439-708) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RUFY1
Gene ID: 80230
Uniprot ID: RUFY1_HUMAN
Immunogen Region: 439-708
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 439-708 of human RUFY1 (NP_079434.3).
Immunogen Sequence: EKDTHEKQDTLVALRQQLEE VKAINLQMFHKAQNAESSLQ QKNEAITSFEGKTNQVMSSM KQMEERLQHSERARQGAEER SHKLQQELGGRIGALQLQLS QLHEQCSSLEKELKSEKEQR QALQRELQHEKDTSSLLRME LQQVEGLKKELRELQDEKAE LQKICEEQEQALQEMGLHLS QSKLKMEDIKEVNQALKGHA WLKDDEATHCRQCEKEFSIS RRKHHCRNCGHIFCNTCSS
Tissue Specificity Broadly expressed, with highest levels in lung, testis, kidney and brain.
Post Translational Modifications Phosphorylation on Tyr-389 and/or Tyr-400 is required for interaction with BMX and endosomal targeting.
Function Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in early endosomal trafficking.
Protein Name Run And Fyve Domain-Containing Protein 1
Fyve-Finger Protein Eip1
La-Binding Protein 1
Rab4-Interacting Protein
Zinc Finger Fyve Domain-Containing Protein 12
Database Links Reactome: R-HSA-1660499
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Run And Fyve Domain-Containing Protein 1 antibody
Anti-Fyve-Finger Protein Eip1 antibody
Anti-La-Binding Protein 1 antibody
Anti-Rab4-Interacting Protein antibody
Anti-Zinc Finger Fyve Domain-Containing Protein 12 antibody
Anti-RUFY1 antibody
Anti-RABIP4 antibody
Anti-ZFYVE12 antibody

Information sourced from Uniprot.org

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