• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g RRM2 antibody (STJ27212). Western blot was performed from the immunoprecipitate using RRM2 antibody (STJ27212) at a dilution of 1:1000.
  • Immunofluorescence analysis of U2OS cells using RRM2 Rabbit polyclonal antibody (STJ27212) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of RRM2 in paraffin-embedded human lung cancer using RRM2 Rabbit polyclonal antibody (STJ27212) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RRM2 in paraffin-embedded human colon carcinoma using RRM2 Rabbit polyclonal antibody (STJ27212) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from HeLa cells, using RRM2 Rabbit polyclonal antibody (STJ27212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RRM2 antibody (1-389) (STJ27212)

SKU:
STJ27212

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RRM2 (1-389) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RRM2
Gene ID: 6241
Uniprot ID: RIR2_HUMAN
Immunogen Region: 1-389
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-389 of human RRM2 (NP_001025.1).
Immunogen Sequence: MLSLRVPLAPITDPQQLQLS PLKGLSLVDKENTPPALSGT RVLASKTARRIFQEPTEPKT KAAAPGVEDEPLLRENPRRF VIFPIEYHDIWQMYKKAEAS FWTAEEVDLSKDIQHWESLK PEERYFISHVLAFFAASDGI VNENLVERFSQEVQITEARC FYGFQIAMENIHSEMYSLLI DTYIKDPKEREFLFNAIETM PCVKKKADWALRWIGDKEAT YGERVVAFAAVEGIFFSGS
Post Translational Modifications Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. Phosphorylated on Thr-33 by CDK1 and CDK2.predominantly in G2 and M phase. Ubiquitinated by the SCF(CCNF) E3 ubiquitin-protein ligase complex.leading to its degradation by the proteasome.
Function Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
Protein Name Ribonucleoside-Diphosphate Reductase Subunit M2
Ribonucleotide Reductase Small Chain
Ribonucleotide Reductase Small Subunit
Database Links Reactome: R-HSA-499943
Reactome: R-HSA-69205
Reactome: R-HSA-8953750
Cellular Localisation Cytoplasm
Nucleus
Localized To The Cytoplasm In S Phase Cells
May Localize To The Nucleus In G2 Phase Cells
Alternative Antibody Names Anti-Ribonucleoside-Diphosphate Reductase Subunit M2 antibody
Anti-Ribonucleotide Reductase Small Chain antibody
Anti-Ribonucleotide Reductase Small Subunit antibody
Anti-RRM2 antibody
Anti-RR2 antibody

Information sourced from Uniprot.org

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