| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RPTOR (888-1013) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RPTOR |
| Gene ID: |
57521 |
| Uniprot ID: |
RPTOR_HUMAN |
| Immunogen Region: |
888-1013 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 888-1013 of human RPTOR (NP_065812.1). |
| Immunogen Sequence: |
LTNDVAKQPVSRDLPSGRPG TTGPAGAQYTPHSHQFPRTR KMFDKGPEQTADDADDAAGH KSFISATVQTGFCDWSARYF AQPVMKIPEEHDLESQIRKE REWRFLRNSRVRRQAQQVIQ KGITRL |
| Tissue Specificity | Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. Isoform 3: Widely expressed, with highest levels in nasal mucosa and pituitary and lowest in spleen. |
| Post Translational Modifications | Insulin-stimulated phosphorylation at Ser-863 by MTOR and MAPK8 regulates mTORC1 activity. Phosphorylated at Ser-863 by NLK in response to stress, disrupting the interaction with small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD), thereby preventing lysosome recruitment and activation of the mTORC1 complex. Osmotic stress also induces phosphorylation at Ser-696, Thr-706 and Ser-863 by MAPK8. Ser-863 phosphorylation is required for phosphorylation at Ser-855 and Ser-859. In response to nutrient limitation, phosphorylated at Ser-722 and Ser-792 by AMPK.phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1, which stimulates mTORC1 activity. Phosphorylation at Ser-791 by PKA downstream of cAMP inhibits the mTORC1 complex. Phosphorylated at Ser-877 by TBK1, leading to negative regulation of the mTORC1 complex. Acetylation at Lys-1097 by EP300/p300 in response to leucine metabolite acetyl-coA promotes its activity, leading to activation of the mTORC1 complex. Acetylation is decreased in response to fasting. Ubiquitinated, leading to its degradation by the proteasome. Deubiquitinated by OTUB1 via a non-catalytic mechanism. Ubiquitinated by an E3 ubiquitin ligase complex containing VHL. |
| Function | Component of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth. In response to nutrients, growth factors or amino acids, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy. The mTORC1 complex is inhibited in response to starvation and amino acid depletion. Within the mTORC1 complex, RPTOR acts both as a molecular adapter, which (1) mediates recruitment of mTORC1 to lysosomal membranes via interaction with small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD), and a (2) substrate-specific adapter, which promotes substrate specificity by binding to TOS motif-containing proteins and direct them towards the active site of the MTOR kinase domain for phosphorylation. mTORC1 complex regulates many cellular processes, such as odontoblast and osteoclast differentiation or neuronal transmission. mTORC1 complex in excitatory neuronal transmission is required for the prosocial behavior induced by the psychoactive substance lysergic acid diethylamide (LSD). |
| Protein Name | Regulatory-Associated Protein Of MtorRaptorP150 Target Of RapamycinTor-Scaffold Protein |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-165159Reactome: R-HSA-166208Reactome: R-HSA-3371571Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-8943724Reactome: R-HSA-9639288 |
| Cellular Localisation | Lysosome MembraneCytoplasmCytoplasmic GranuleTargeting To Lysosomes Depends On Amino Acid Availability: Recruited To Lysosome Membranes Via Interaction With Gtp-Bound Form Of Raga/Rraga (Or Ragb/Rragb) In Complex With The Gdp-Bound Form Of Ragc/Rragc (Or Ragd/Rragd)Promoting Recruitment Of Mtorc1 To The LysosomesIn Arsenite-Stressed CellsAccumulates In Stress Granules When Associated With Spag5 And Association With Lysosomes Is Drastically Decreased |
| Alternative Antibody Names | Anti-Regulatory-Associated Protein Of Mtor antibodyAnti-Raptor antibodyAnti-P150 Target Of Rapamycin antibodyAnti-Tor-Scaffold Protein antibodyAnti-RPTOR antibodyAnti-KIAA1303 antibodyAnti-RAPTOR antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
