• Immunofluorescence analysis of HeLa cells using RPLP0 antibody (STJ27503). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using RPLP0 antibody (STJ27503) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPLP0 antibody (1-317) (STJ27503)

SKU:
STJ27503

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPLP0 (1-317) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPLP0
Gene ID: 6175
Uniprot ID: RLA0_HUMAN
Immunogen Region: 1-317
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human RPLP0 (NP_000993.1).
Immunogen Sequence: MPREDRATWKSNYFLKIIQL LDDYPKCFIVGADNVGSKQM QQIRMSLRGKAVVLMGKNTM MRKAIRGHLENNPALEKLLP HIRGNVGFVFTKEDLTEIRD MLLANKVPAAARAGAIAPCE VTVPAQNTGLGPEKTSFFQA LGITTKISRGTIEILSDVQL IKTGDKVGASEATLLNMLNI SPFSFGLVIQQVFDNGSIYN PEVLDITEETLHSRFLEGVR NVASVCLQIGYPTVASVPH
Post Translational Modifications Ubiquitinated at Lys-264 by RNF14 and RNF25 in response to ribosome collisions (ribosome stalling).
Function Ribosomal protein P0 is the functional equivalent of E.coli protein L10.
Protein Name Large Ribosomal Subunit Protein Ul10
60s Acidic Ribosomal Protein P0
60s Ribosomal Protein L10e
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-8950505
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Nucleus
Cytoplasm
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ul10 antibody
Anti-60s Acidic Ribosomal Protein P0 antibody
Anti-60s Ribosomal Protein L10e antibody
Anti-RPLP0 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance