• Immunohistochemistry analysis of RPL31 in paraffin-embedded mouse spleen using RPL31 Rabbit polyclonal antibody (STJ119619) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RPL31 in paraffin-embedded rat kidney using RPL31 Rabbit polyclonal antibody (STJ119619) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RPL31 Rabbit polyclonal antibody (STJ119619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPL31 antibody (5-105) (STJ119619)

SKU:
STJ119619

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RPL31 (5-105) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RPL31
Gene ID: 6160
Uniprot ID: RL31_HUMAN
Immunogen Region: 5-105
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 5-105 of human RPL31 (NP_000984.1).
Immunogen Sequence: KKGGEKKKGRSAINEVVTRE YTINIHKRIHGVGFKKRAPR ALKEIRKFAMKEMGTPDVRI DTRLNKAVWAKGIRNVPYRI RVRLSRKRNEDEDSPNKLYT L
Function Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.
Protein Name Large Ribosomal Subunit Protein El31
60s Ribosomal Protein L31
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-156902
Reactome: R-HSA-1799339
Reactome: R-HSA-192823
Reactome: R-HSA-2408557
Reactome: R-HSA-6791226
Reactome: R-HSA-72689
Reactome: R-HSA-72706
Reactome: R-HSA-72764
Reactome: R-HSA-9010553
Reactome: R-HSA-9633012
Reactome: R-HSA-975956
Reactome: R-HSA-975957
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein El31 antibody
Anti-60s Ribosomal Protein L31 antibody
Anti-RPL31 antibody

Information sourced from Uniprot.org

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